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KMID : 0350519930460031113
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Journal of Catholic Medical College
1993 Volume.46 No. 3 p.1113 ~ p.1123
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The Effect of Luteinizing Hormone Releasing Hormone Ethylamide on Testicular Function in the Cryptorchid Rat
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Abstract
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Cryptorchidism is the most common disorder of sexual differentiation that occurs in man. The etiology of cryptorchidism is felt to be multifactorial, with one proposed mechanism being abnormal hypothalamic-pituitary stimulation of the gonad.
It is generally accepted that the patient with cryptorchidism experienced reduced fertility potential. Nevetheless, the optimal form of treatment and the effects of treatment remain unclear. In an attempt to provide some insight into the
mechanisms
reponsible for this important event experimental observation and clinical correlations were made.
To assess the effects of an Luteinizing Hormone Releasing Hormone(LHRH) agonist on the function of the cryptorchid testis, an animal model was created using prepubertal male Sprague-Dawley rats(21 days old).
The animal models were divided into five groups : 1) sham operated(control) ; 2) cryptorchid ; 3) cryptorchid + LHRH ; 4) cryptorchid + orchiopxed, and 5) cryptorchid + LHRH + orchiopexed groups. The effects of each treatment were assessed in
terms
of
body weight, testicular weight, serum level of testosterone, testicular histology(spermatogenesis) and fertility rate.
@ES The results were as follows :
@EN 1. The body werght was not altered in cryptorchid group compared to control, but the testicular weight was significantly decreased in cryptorchid group and cryptorchid + LHRH group compared to control(P<0.05). The cryptorchid + LHRH group
resulted
in a significant decrease in the body weight and the testicular weight relative to cryptorchid group(P<0.05). The cryptorchid +LHRH +orchiopexed group was significantly decreased in the body weight and testicular weight compared to cryptorchid +
orchiopexed group(P<0.05).
2. Serum testosterons level was significantly decreased in cryptorchid group and cryptorchid +LHRH group(1.67±0.14 ng/ml, 1.37±0.36 ng/ml) compared to control(3.35±0.42 ng/ml) (P<0.05), respectively. The testosternoe level in
cryptorchid+orchiopexed
group(3.49±0.24 ng/ml) was similar to control. But a marked increase of serum tesstosterone level was observed in cryptochid + LHRH + orchiopexed group(6.51±0.27 ng/ml) compared to control and cryptorechid + orchiopexed group(P<0.05).
3. Activity of spermatogenesis was merkedly recoverred in cryptorchid + LHRH + orchiopexed group(tuble number 30.8±2.7, spermatogenic index 3.4) than that treated with either cryptorchid + LHRH group(38.7±3.6, 2.1) or cryptorchid + orchiopexed
group(45.2±5.6, 1.9) (P<0.05), respectively, but they were not reach to the levels of control(23.4±2.4, 4.5).
4. Fertility rate was significantly improved in cryptorchid + LHRH + orchiopexed group(55.5%) than cryptorchid + orchiopexed group(36.6%) (P<0.05).
These results suggest that experimental cryptorchidism decreased weight of testis and impaired reproductive function, which were not restored by orchiopexy alone. Treatment of cryptorchid testis prior to orchiopexy with an LHRH agonist may
enhance
germ
cell division and ultimate germ cell number, thus improving fertility potential. But further studies seem to be necessary in order to define the effects of LHRH agonists-the timing, dosage and duration of administration-on the cryptorchidism in
more
detail.
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KEYWORD
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